Application

Research CategoryStem Cell Research

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

KGF/FGF-7 is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. KGF/FGF-7 is a mitogen factor specific for epithelial cells and keratinocytes. KGF/FGF-7 signals through FGFR 2b. KGF/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing.
Recombinant human KGF/FGF-7 is an 18.9 kDa protein consisting of 163 amino acid residues.

Physical form

Product is presented in 15 mM Sodium Phosphate pH 7.0 + 0.25 M NaCl and is filtered through a 0.2 micron filter before lyophilization.

Quality

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is ≤ 10 ng/ml
corresponding to a specific activity of ≥ 1 x 10^5 units/mg.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in 1x PBS to a concentration of 0.5-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.